Symplekin

From Proteopedia

Human symplekin N terminal (grey) complex with RNA polymerase II subunit A phosphatase C terminal (green) and phosphate ion (PDB code 3o2s) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Symplekin (SMPK) is a scaffold protein recruiting regulatory factors to the multimolecular complex involved in histone mRNA 3’-end processing. SMPK is involved in pre-mRNA polyadenylation. It enhances the activity of RNA polymerase II subunit A C terminal phosphatase (Ssu72)[1]. Relevance Overexpression of SMPK promotes tumorigenicity in the colon[2].

3D Structures of symplekin

Updated on 15-November-2020

3o2t, 3odr – hSMPK N terminal – human
3ods – hSMPK N terminal (mutant)
3o2s – hSMPK N terminal + RNA polymerase II subunit A C terminal phosphatase
4h3h, 3o2q, 4h3k – hSMPK N terminal + RNA polymerase II subunit A C terminal phosphatase + phosphorylated peptide
6v4x – hSMPK inhistone pre-mRNA 3’-end processing machinery – Cryo EM
3gs3 – DmSMPK N terminal – Drosophila melanogaster
4imi, 4imj, 4ygx, 6npw – DmSMPK + SSU72 + phosphorylated peptide

References

1. ↑ Keon BH, Schafer S, Kuhn C, Grund C, Franke WW. Symplekin, a novel type of tight junction plaque protein. J Cell Biol. 1996 Aug;134(4):1003-18. PMID:8769423
2. ↑ . PMID:216315890657