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Poly(A) polymerase binds specifically to ATP and adds it the end of a mRNA chain. This structure contains an oligo(A) polynucleotide with 5 nucleotides, an ATP molecule, and a magnesium ion. … In the , the enzyme is shown as a blue backbone, the RNA chain in yellow, the ATP in red, the Mg++ in green, and ALA154 in magenta. Several mechanisms are used to achieve the specificity for ATP. The Mg++ is coordinated by , and the Mg++ coordinates with the phosphates of ATP, positioning the nucleotide in the active site. The adenine base is sandwiched between the . (more...)
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