Sandbox Reserved 564
From Proteopedia
This Sandbox is Reserved from 05/22/2012, through 07/22/2012 for use in the course "BIOL 414" taught by Greg Buhrman at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 551 through Sandbox Reserved 590. |
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Hi Mike, Here is your very own Proteopedia Page for pdb code: 1M8Q. Your presentation is scheduled for: June 15.
Have Fun!
Greg Buhrman
Contents |
Molecular Models of Averaged Rigor Crossbridges from Tomograms of Insect Flight Muscle
Electron tomography, correspondence analysis, molecular model building, and real-space refinement provide detailed 3-D structures for in situ myosin crossbridges in the nucleotide-free state (rigor), thought to represent the end of the power stroke. Unaveraged tomograms from a 25-nm longitudinal section of insect flight muscle preserved native structural variation. Recurring crossbridge motifs that repeat every 38.7 nm along the actin filament were extracted from the tomogram and classified by correspondence analysis into 25 class averages, which improved the signal to noise ratio. Models based on the atomic structures of actin and of myosin subfragment 1 were rebuilt to fit 11 class averages. A real-space refinement procedure was applied to quantitatively fit the reconstructions and to minimize steric clashes between domains introduced during the fitting. These combined procedures show that no single myosin head structure can fit all the in situ crossbridges. The validity of the approach is supported by agreement of these atomic models with fluorescent probe data from vertebrate muscle as well as with data from regulatory light chain crosslinking between heads of smooth muscle heavy meromyosin when bound to actin.
Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle., Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA, J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1m8q is a 26 chain structure of Actin and Myosin with sequence from Gallus gallus and Oryctolagus cuniculus. Full crystallographic information is available from OCA.
See Also
Reference
- Chen LF, Winkler H, Reedy MK, Reedy MC, Taylor KA. Molecular modeling of averaged rigor crossbridges from tomograms of insect flight muscle. J Struct Biol. 2002 Apr-May;138(1-2):92-104. PMID:12160705