5woa

From Proteopedia

Crystal Structure of Transient Receptor Potential (TRP) channel TRPV6* in the presence of Gadolinium

PDB ID 5woa

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Structural highlights

5woa is a 1 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.899Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRPV6_RAT Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).[UniProtKB:Q91WD2][UniProtKB:Q9H1D0]^[1]

Publication Abstract from PubMed

Tetrameric ion channels have either swapped or non-swapped arrangements of the S1-S4 and pore domains. Here we show that mutations in the transmembrane domain of TRPV6 can result in conversion from a domain-swapped to non-swapped fold. These results reveal structural determinants of domain swapping and raise the possibility that a single ion channel subtype can fold into either arrangement in vivo, affecting its function in normal or disease states.

Swapping of transmembrane domains in the epithelial calcium channel TRPV6.,Singh AK, Saotome K, Sobolevsky AI Sci Rep. 2017 Sep 6;7(1):10669. doi: 10.1038/s41598-017-10993-9. PMID:28878326^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yue L, Peng JB, Hediger MA, Clapham DE. CaT1 manifests the pore properties of the calcium-release-activated calcium channel. Nature. 2001 Apr 5;410(6829):705-9. PMID:11287959 doi:http://dx.doi.org/10.1038/35070596
↑ Singh AK, Saotome K, Sobolevsky AI. Swapping of transmembrane domains in the epithelial calcium channel TRPV6. Sci Rep. 2017 Sep 6;7(1):10669. doi: 10.1038/s41598-017-10993-9. PMID:28878326 doi:http://dx.doi.org/10.1038/s41598-017-10993-9