Structural highlights
Function
Q74849_9HIV1
Publication Abstract from PubMed
HIV-1 envelope spike (Env) is a type I membrane protein that mediates viral entry. We used nuclear magnetic resonance to determine an atomic structure of the transmembrane (TM) domain of HIV-1 Env reconstituted in bicelles that mimic a lipid bilayer. The TM forms a well-ordered trimer that protects a conserved membrane-embedded arginine. An amino-terminal coiled-coil and a carboxyl-terminal hydrophilic core stabilize the trimer. Individual mutations of conserved residues did not disrupt the TM trimer and minimally affected membrane fusion and infectivity. Major changes in the hydrophilic core, however, altered the antibody sensitivity of Env. These results show how a TM domain anchors, stabilizes, and modulates a viral envelope spike and suggest that its influence on Env conformation is an important consideration for HIV-1 immunogen design.
Structural basis for membrane anchoring of HIV-1 envelope spike.,Dev J, Park D, Fu Q, Chen J, Ha HJ, Ghantous F, Herrmann T, Chang W, Liu Z, Frey G, Seaman MS, Chen B, Chou JJ Science. 2016 Jul 8;353(6295):172-5. doi: 10.1126/science.aaf7066. Epub 2016 Jun , 23. PMID:27338706[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Dev J, Park D, Fu Q, Chen J, Ha HJ, Ghantous F, Herrmann T, Chang W, Liu Z, Frey G, Seaman MS, Chen B, Chou JJ. Structural basis for membrane anchoring of HIV-1 envelope spike. Science. 2016 Jul 8;353(6295):172-5. doi: 10.1126/science.aaf7066. Epub 2016 Jun , 23. PMID:27338706 doi:http://dx.doi.org/10.1126/science.aaf7066