3o3c

From Proteopedia

Jump to: navigation, search
3o3c, resolution 3.51Å ()
Ligands: ,
Gene: GSY2, YLR258W, L8479.8 (Saccharomyces cerevisiae)
Activity: Glycogen(starch) synthase, with EC number 2.4.1.11
Related: 1rzu, 2bis


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

Glycogen synthase basal state UDP complex

Publication Abstract from PubMed

Regulation of the storage of glycogen, one of the major energy reserves, is of utmost metabolic importance. In eukaryotes, this regulation is accomplished through glucose-6-phosphate levels and protein phosphorylation. Glycogen synthase homologs in bacteria and archaea lack regulation, while the eukaryotic enzymes are inhibited by protein kinase mediated phosphorylation and activated by protein phosphatases and glucose-6-phosphate binding. We determined the crystal structures corresponding to the basal activity state and glucose-6-phosphate activated state of yeast glycogen synthase-2. The enzyme is assembled into an unusual tetramer by an insertion unique to the eukaryotic enzymes, and this subunit interface is rearranged by the binding of glucose-6-phosphate, which frees the active site cleft and facilitates catalysis. Using both mutagenesis and intein-mediated phospho-peptide ligation experiments, we demonstrate that the enzyme's response to glucose-6-phosphate is controlled by Arg583 and Arg587, while four additional arginine residues present within the same regulatory helix regulate the response to phosphorylation.

Structural basis for glucose-6-phosphate activation of glycogen synthase., Baskaran S, Roach PJ, Depaoli-Roach AA, Hurley TD, Proc Natl Acad Sci U S A. 2010 Sep 27. PMID:20876143

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Function

[GYS2_YEAST] Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

About this Structure

3o3c is a 4 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

  • Baskaran S, Roach PJ, Depaoli-Roach AA, Hurley TD. Structural basis for glucose-6-phosphate activation of glycogen synthase. Proc Natl Acad Sci U S A. 2010 Sep 27. PMID:20876143 doi:10.1073/pnas.1006340107

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools