3i4m

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3i4m, resolution 3.70Å ()
Ligands: ,
Non-Standard Residues: ,
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Related: 3i4n
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Contents

8-oxoguanine containing RNA polymerase II elongation complex D

Publication Abstract from PubMed

Structure-function analysis has revealed the mechanism of yeast RNA polymerase II transcription at 8-oxoguanine (8-oxoG), the major DNA lesion resulting from oxidative stress. When polymerase II encounters 8-oxoG in the DNA template strand, it can misincorporate adenine, which forms a Hoogsteen bp with 8-oxoG at the active center. This requires rotation of the 8-oxoG base from the standard anti- to an uncommon syn-conformation, which likely occurs during 8-oxoG loading into the active site. The misincorporated adenine escapes intrinsic proofreading, resulting in transcriptional mutagenesis that is observed directly by mass spectrometric RNA analysis.

Molecular basis of transcriptional mutagenesis at 8-oxoguanine., Damsma GE, Cramer P, J Biol Chem. 2009 Nov 13;284(46):31658-63. Epub 2009 Sep 16. PMID:19758983

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3i4m is a 15 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Damsma GE, Cramer P. Molecular basis of transcriptional mutagenesis at 8-oxoguanine. J Biol Chem. 2009 Nov 13;284(46):31658-63. Epub 2009 Sep 16. PMID:19758983 doi:M109.022764

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