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3eyx

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3eyx, resolution 2.04Å ()
Ligands: , ,
Gene: NCE103 (Saccharomyces cerevisiae)
Activity: Carbonate dehydratase, with EC number 4.2.1.1
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of Carbonic Anhydrase Nce103 from Saccharomyces cerevisiae

Publication Abstract from PubMed

BACKGROUND: The carbonic anhydrases (CAs) are involved in inorganic carbon utilization. They have been classified into six evolutionary and structural families: alpha-, beta-, gamma-, delta-, epsilon-, zeta- CAs, with beta-CAs present in higher plants, algae and prokaryotes. The yeast Saccharomyces cerevisiae encodes a single copy of beta-CA Nce103/YNL036W. RESULTS: We determined the crystal structure of Nce103 in complex with a substrate analog at 2.04 A resolution. It assembles as a homodimer, with the active site located at the interface between two monomers. At the bottom of the substrate pocket, a zinc ion is coordinated by the three highly conserved residues Cys57, His112 and Cys115 in addition to a water molecule. Residues Asp59, Arg61, Gly111, Leu102, Val80, Phe75 and Phe97 form a tunnel to the bottom of the active site which is occupied by a molecule of the substrate analog acetate. Activity assays of full length and two truncated versions of Nce103 indicated that the N-terminal arm is indispensable. CONCLUSION: The quaternary structure of Nce103 resembles the typical plant type beta-CAs of known structure, with an N-terminal arm indispensable for the enzymatic activity. Comparative structure analysis enables us to draw a possible tunnel for the substrate to access the active site which is located at the bottom of a funnel-shaped substrate pocket.

Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103., Teng YB, Jiang YL, He YX, He WW, Lian FM, Chen Y, Zhou CZ, BMC Struct Biol. 2009 Oct 24;9:67. PMID:19852838

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3eyx is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Teng YB, Jiang YL, He YX, He WW, Lian FM, Chen Y, Zhou CZ. Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103. BMC Struct Biol. 2009 Oct 24;9:67. PMID:19852838 doi:10.1186/1472-6807-9-67

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