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3dc5

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3dc5, resolution 1.70Å ()
Ligands: ,
Gene: sod-3, C08A9.1 (Caenorhabditis elegans)
Activity: Superoxide dismutase, with EC number 1.15.1.1
Related: 3dc6
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal Structure of a manganese superoxide dismutases from Caenorhabditis elegans

Publication Abstract from PubMed

Caenorhabditis elegans expresses two manganese superoxide dismutase enzymes (MnSOD-2 and MnSOD-3) that are targeted to the mitochondrion. MnSOD-2 is constitutively expressed, while synthesis of MnSOD-3 is inducible. The structures of these two mononuclear metalloenzymes have been determined to 1.8 and 1.7 A resolution, respectively. Pink crystals formed in space group P4(1)2(1)2 for each, with unit-cell parameters a = b = 81.0, c = 137.4 A for MnSOD-2 and a = b = 81.8, c = 136.0 A for MnSOD-3. The final structure of MnSOD-3 was refined to R = 21.6% and R(free) = 26.2% at 293 K, and R = 18.9% and R(free) = 22.6% at 100 K, while that of MnSOD-2 was refined to R = 16.9% and R(free) = 20.1% at 100 K. The asymmetric unit cell is comprised of two subunits. The resulting structures are very similar to that of human MnSOD and form a tetramer corresponding to a dimer of dimers. The subunit interface between dimers is comprised of two four-helix bundles that stabilize the biologically significant homotetramer.

Purification, crystallization and X-ray structures of the two manganese superoxide dismutases from Caenorhabditis elegans., Trinh CH, Hunter T, Stewart EE, Phillips SE, Hunter GJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt, 12):1110-4. Epub 2008 Nov 28. PMID:19052361

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3dc5 is a 2 chain structure with sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.

See Also

Reference

  • Trinh CH, Hunter T, Stewart EE, Phillips SE, Hunter GJ. Purification, crystallization and X-ray structures of the two manganese superoxide dismutases from Caenorhabditis elegans. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Dec 1;64(Pt, 12):1110-4. Epub 2008 Nov 28. PMID:19052361 doi:10.1107/S1744309108037056

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