First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
3cfp
From Proteopedia
| 3cfp, resolution 2.50Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||||
| Non-Standard Residues: | |||||||||
| Gene: | 43 (Enterobacteria phage RB69) | ||||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||||
| Related: | 1ih7, 1ig9, 3cfo, 3cfr | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Structure of the replicating complex of a POL Alpha family DNA Polymerase, ternary complex 1
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3cfp is a 3 chain structure with sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.
See Also
Reference
- Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA. Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69. Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
Categories: DNA-directed DNA polymerase | Enterobacteria phage rb69 | Klimenko, D. | Konigsberg, W H. | Steitz, T A. | Wang, J. | Wang, M. | Catalytic complex | Dna polymerase | Dna replication | Dna-binding | Dna-directed dna polymerase | Exonuclease | Fidelity | Hydrolase | Nuclease | Nucleotidyltransferase | Transferase | Transferase-dna complex | Two metal ion mechanism
