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2zjp
From Proteopedia
Contents |
Thiopeptide antibiotic Nosiheptide bound to the large ribosomal subunit of Deinococcus radiodurans
The thiopeptide class of antibiotics targets the GTPase-associated center (GAC) of the ribosome to inhibit translation factor function. Using X-ray crystallography, we have determined the binding sites of thiostrepton (Thio), nosiheptide (Nosi), and micrococcin (Micro), on the Deinococcus radiodurans large ribosomal subunit. The thiopeptides, by binding within a cleft located between the ribosomal protein L11 and helices 43 and 44 of the 23S rRNA, overlap with the position of domain V of EF-G, thus explaining how this class of drugs perturbs translation factor binding to the ribosome. The presence of Micro leads to additional density for the C-terminal domain (CTD) of L7, adjacent to and interacting with L11. The results suggest that L11 acts as a molecular switch to control L7 binding and plays a pivotal role in positioning one L7-CTD monomer on the G' subdomain of EF-G to regulate EF-G turnover during protein synthesis.
Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin., Harms JM, Wilson DN, Schluenzen F, Connell SR, Stachelhaus T, Zaborowska Z, Spahn CM, Fucini P, Mol Cell. 2008 Apr 11;30(1):26-38. PMID:18406324
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2zjp is a 31 chain structure of Ribosomal protein L11, Ribosomal protein L13, Ribosomal protein L14, Ribosomal protein L15, Ribosomal protein L16, Ribosomal protein L17, Ribosomal protein L18, Ribosomal protein L19, Ribosomal protein L2, Ribosomal protein L20, Ribosomal protein L21, Ribosomal protein L22, Ribosomal protein L23, Ribosomal protein L24, Ribosomal protein L25, Ribosomal protein L27, Ribosomal protein L28, Ribosomal protein L29, Ribosomal protein L3, Ribosomal protein L30, Ribosomal protein L32, Ribosomal protein L33, Ribosomal protein L34, Ribosomal protein L35, Ribosomal protein L36, Ribosomal protein L4, Ribosomal protein L5, Ribosomal protein L6 and Ribosome with sequence from Deinococcus radiodurans and Streptomyces actuosus. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L11
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L28
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosome
Reference
- Harms JM, Wilson DN, Schluenzen F, Connell SR, Stachelhaus T, Zaborowska Z, Spahn CM, Fucini P. Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin. Mol Cell. 2008 Apr 11;30(1):26-38. PMID:18406324 doi:S1097-2765(08)00044-0
Categories: Deinococcus radiodurans | Streptomyces actuosus | Connell, S R. | Fucini, P. | Harms, J M. | Schluenzen, F. | Spahn, C M.T. | Stachelhaus, T. | Wilson, D N. | Zaborowska, Z. | Antibacterial | Antibiotic | L11 | Nosiheptide | Ribosome | Ribosome-antibiotic complex | Rna-binding | S50 | Thiazole | Thiopeptide | Translation regulation | Zinc-finger
