2wvw

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2wvw, resolution 9.00Å ()
Activity: Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39
Related: 1rsc, 1rbl
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Cryo-EM structure of the RbcL-RbcX complex

Publication Abstract from PubMed

Form I Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase), a complex of eight large (RbcL) and eight small (RbcS) subunits, catalyses the fixation of atmospheric CO(2) in photosynthesis. The limited catalytic efficiency of Rubisco has sparked extensive efforts to re-engineer the enzyme with the goal of enhancing agricultural productivity. To facilitate such efforts we analysed the formation of cyanobacterial form I Rubisco by in vitro reconstitution and cryo-electron microscopy. We show that RbcL subunit folding by the GroEL/GroES chaperonin is tightly coupled with assembly mediated by the chaperone RbcX(2). RbcL monomers remain partially unstable and retain high affinity for GroEL until captured by RbcX(2). As revealed by the structure of a RbcL(8)-(RbcX(2))(8) assembly intermediate, RbcX(2) acts as a molecular staple in stabilizing the RbcL subunits as dimers and facilitates RbcL(8) core assembly. Finally, addition of RbcS results in RbcX(2) release and holoenzyme formation. Specific assembly chaperones may be required more generally in the formation of complex oligomeric structures when folding is closely coupled to assembly.

Coupled chaperone action in folding and assembly of hexadecameric Rubisco., Liu C, Young AL, Starling-Windhof A, Bracher A, Saschenbrecker S, Rao BV, Rao KV, Berninghausen O, Mielke T, Hartl FU, Beckmann R, Hayer-Hartl M, Nature. 2010 Jan 14;463(7278):197-202. PMID:20075914

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2wvw is a 24 chain structure with sequence from Anabaena sp. ca and Synechococcus elongatus. Full crystallographic information is available from OCA.

See Also

Reference

  • Liu C, Young AL, Starling-Windhof A, Bracher A, Saschenbrecker S, Rao BV, Rao KV, Berninghausen O, Mielke T, Hartl FU, Beckmann R, Hayer-Hartl M. Coupled chaperone action in folding and assembly of hexadecameric Rubisco. Nature. 2010 Jan 14;463(7278):197-202. PMID:20075914 doi:10.1038/nature08651

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