2jj7

From Proteopedia

Crystal structure of the HlyIIR mutant protein with residues 170-185 substituted by alanine

PDB ID 2jj7

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Structural highlights

2jj7 is a 2 chain structure with sequence from Bacillus cereus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q7X506_BACCE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

HlyIIR is the negative transcriptional regulator of the hemolysin II gene from Bacillus cereus. Previous X-ray studies showed that HlyIIR contains a disordered loop (a.a. 170-185) located within the C-terminal domain near dimerization interface. To understand the influence of this region on HlyIIR properties and for a potential improvement in the crystallogenesis of the HlyIIR, we constructed a mutant of HlyIIR in which this disordered region is substituted by a single alanine residue. Biochemical analysis of the mutant indicated that it still forms a dimer but the DNA-binding activity is lost. HlyIIR mutant displayed improved crystallization properties and its structure was determined by X-ray crystallography at 2.1 A resolution. Unexpectedly, the structure shows that the HlyIIR mutant forms an alternative dimer with subunits rotated by 160 degrees. Moreover, there are also changes in the conformation of individual subunits. These dramatic structural rearrangements are caused by changes in the conformation of the segment Pro161-Ser169. We conclude that correct conformation of this segment is principal for maintaining the structure and activity of HlyIIR.

[Contraction of the disordered loop located within C-terminal domain of the transcriptional regulator HlyIIR causes its structural rearrangement],Kovalevskii OV, Antson AA, Solonin AS Mol Biol (Mosk). 2009 Jan-Feb;43(1):126-35. PMID:19334535^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kovalevskii OV, Antson AA, Solonin AS. [Contraction of the disordered loop located within C-terminal domain of the transcriptional regulator HlyIIR causes its structural rearrangement] Mol Biol (Mosk). 2009 Jan-Feb;43(1):126-35. PMID:19334535