Structural highlights
Function
DSZB_RHOSG Catalyzes the third and final step of the '4S' desulfurization pathway that removes covalently bound sulfur from dibenzothiophene (DBT) without breaking carbon-carbon bonds. Oxidizes 2-(2'-hydroxyphenyl)benzene sulphinate (HBPS) to 2-hydroxybiphenyl (HBP) plus sulfite (PubMed:7961424, PubMed:7574582, PubMed:9634856, PubMed:9308179, PubMed:31545606). The rate-limiting step of the '4S' desulfurization pathway (PubMed:9308179, PubMed:31545606). The pathway substrate specificity has been augmented using mutagenesis, however no mutations allowed use of alkylated thiophenes (PubMed:11823208).[1] [2] [3] [4] [5] [6]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Arensdorf JJ, Loomis AK, DiGrazia PM, Monticello DJ, Pienkos PT. Chemostat approach for the directed evolution of biodesulfurization gain-of-function mutants. Appl Environ Microbiol. 2002 Feb;68(2):691-8. doi: 10.1128/AEM.68.2.691-698.2002. PMID:11823208 doi:http://dx.doi.org/10.1128/AEM.68.2.691-698.2002
- ↑ Yu Y, Mills LC, Englert DL, Payne CM. Inhibition Mechanisms of Rhodococcus Erythropolis 2'-Hydroxybiphenyl-2-sulfinate Desulfinase (DszB). J Phys Chem B. 2019 Oct 31;123(43):9054-9065. PMID:31545606 doi:10.1021/acs.jpcb.9b05252
- ↑ Piddington CS, Kovacevich BR, Rambosek J. Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulfurization operon of Rhodococcus sp. strain IGTS8. Appl Environ Microbiol. 1995 Feb;61(2):468-75. PMID:7574582
- ↑ Denome SA, Oldfield C, Nash LJ, Young KD. Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8. J Bacteriol. 1994 Nov;176(21):6707-16. PMID:7961424
- ↑ Oldfield C, Pogrebinsky O, Simmonds J, Olson ES, Kulpa CF. Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968). Microbiology (Reading). 1997 Sep;143 ( Pt 9):2961-2973. doi: , 10.1099/00221287-143-9-2961. PMID:9308179 doi:http://dx.doi.org/10.1099/00221287-143-9-2961
- ↑ Gray KA, Pogrebinsky OS, Mrachko GT, Xi L, Monticello DJ, Squires CH. Molecular mechanisms of biocatalytic desulfurization of fossil fuels. Nat Biotechnol. 1996 Dec;14(13):1705-9. doi: 10.1038/nbt1296-1705. PMID:9634856 doi:http://dx.doi.org/10.1038/nbt1296-1705
