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2cnm
From Proteopedia
| 2cnm, resolution 2.60Å () | |||||||||
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| Ligands: | |||||||||
| Non-Standard Residues: | |||||||||
| Activity: | Ribosomal-protein-alanine N-acetyltransferase, with EC number 2.3.1.128 | ||||||||
| Related: | 2cns, 2cnt | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
RIMI - RIBOSOMAL S18 N-ALPHA-PROTEIN ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE INHIBITOR (CTERM-ARG-ARG-PHE-TYR-ARG-ALA-N-ALPHA-ACETYL-S-COA).
The three ribosomal proteins L7, S5, and S18 are included in the rare subset of prokaryotic proteins that are known to be N(alpha)-acetylated. The GCN5-related N-acetyltransferase (GNAT) protein RimI, responsible for the N(alpha)-acetylation of the ribosomal protein S18, was cloned from Salmonella typhimurium LT2 (RimI(ST)), overexpressed, and purified to homogeneity. Steady-state kinetic parameters for RimI(ST) were determined for AcCoA and a peptide substrate consisting of the first six amino acids of the target protein S18. The crystal structure of RimI(ST) was determined in complex with CoA, AcCoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor. The structures are consistent with a direct nucleophilic addition-elimination mechanism with Glu103 and Tyr115 acting as the catalytic base and acid, respectively. The RimI(ST)-bisubstrate complex suggests that several residues change conformation upon interacting with the N terminus of S18, including Glu103, the proposed active site base, facilitating proton exchange and catalysis.
Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18., Vetting MW, Bareich DC, Yu M, Blanchard JS, Protein Sci. 2008 Oct;17(10):1781-90. Epub 2008 Jul 2. PMID:18596200
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2cnm is a 6 chain structure with sequence from Salmonella enterica subsp. enterica serovar typhimurium str. lt2. Full crystallographic information is available from OCA.
See Also
Reference
- Vetting MW, Bareich DC, Yu M, Blanchard JS. Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18. Protein Sci. 2008 Oct;17(10):1781-90. Epub 2008 Jul 2. PMID:18596200 doi:10.1110/ps.035899.108
Categories: Ribosomal-protein-alanine N-acetyltransferase | Salmonella enterica subsp. enterica serovar typhimurium str. lt2 | Bareich, D C. | Blanchard, J S. | Vetting, M W. | Yu, M. | Acetyltransferase | Acyltransferase | Gcn5-n-acetyltransferase | Gnat | N-alpha acetylation | Ribosomal protein | Transferase
