2b8h

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2b8h, resolution 2.20Å ()
Ligands: , , , , ,
Activity: Exo-alpha-sialidase, with EC number 3.2.1.18
Related: 1a14, 1nmb, 1nmc, 7nn9
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase

Publication Abstract from PubMed

The X-ray structure of influenza virus neuraminidase (NA) isolated from whale, subtype N9, has been determined at 2.2 A resolution and contains a tetrameric protein in the asymmetric unit. In structures of NA determined previously, a calcium ion is observed to coordinate amino acids near the substrate-binding site. In three of the NA monomers determined here this calcium is absent, resulting in structural alterations near the substrate-binding site. These changes affect the conformation of residues that participate in several key interactions between the enzyme and substrate and provide at a molecular level the basis of the structural and functional role of calcium in substrate and inhibitor binding. Several sulfate ions were identified in complex with the protein. These are located in the active site, occupying the space reserved for the substrate (sialic acid) carboxylate, and in positions leading away from the substrate-binding site. These sites offer a new opportunity for the design of inhibitors of influenza virus NA.

Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding., Smith BJ, Huyton T, Joosten RP, McKimm-Breschkin JL, Zhang JG, Luo CS, Lou MZ, Labrou NE, Garrett TP, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):947-52. Epub 2006, Aug 19. PMID:16929094

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

2b8h is a 4 chain structure with sequence from Influenza a virus. Full crystallographic information is available from OCA.

See Also

Reference

  • Smith BJ, Huyton T, Joosten RP, McKimm-Breschkin JL, Zhang JG, Luo CS, Lou MZ, Labrou NE, Garrett TP. Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding. Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):947-52. Epub 2006, Aug 19. PMID:16929094 doi:http://dx.doi.org/10.1107/S0907444906020063

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