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1yuh
From Proteopedia
| 1yuh, resolution 3.00Å () | |
|---|---|
| Ligands: | |
| Domains: | IGc, IGv, V-set |
| Resources: | FirstGlance, OCA, PDBsum, RCSB |
| Coordinates: | save as pdb, mmCIF, xml |
FAB FRAGMENT
Affinity maturation of the immune response to nitrophenol-containing antigens has been extensively investigated. Significant strides made during the past several years with the advent of PCR technology have provided a wealth of biochemical knowledge. Structural investigations of the phenomena have however been limited. We have determined the three-dimensional structure of the Fab fragment of 88C6/12, an anti-4-hydroxy-3-nitrophenyl acetic acid antibody complexed with the immunizing hapten and with a heteroclitic iodinated hapten. The crystallographic structure of the complexes reveals that the binding is stabilized by a number of hydrogen bonds and extensive van der Waals interactions between the hapten and the antibody. In addition, the Fab binding pocket contains a region of positive electrostatic potential well suited for interaction with the predominant resonance form of the nitrophenyl ring system. The observed heteroclicity towards the iodinated hapten is not a direct result of iodine-protein interactions, but results from the enhanced stability in the iodinated ring of the resonance form that binds the antibody. In addition this investigation provides a rationale for the strong preference for the substitution in the heavy chain from the germ-line gene encoded Trp 33 to Leu 33 in the mature anti-nitrophenol response.
Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:8544863
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1YUH is a Protein complex structure. Full crystallographic information is available from OCA.
Reference
Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:8544863
Page seeded by OCA on Mon Jul 28 05:16:11 2008
