First time at Proteopedia? Click on the green links, they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.
1jf9
From Proteopedia
| 1jf9, resolution 2.00Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ligands: | |||||||||
| Gene: | NIFS/CSDB (Escherichia coli) | ||||||||
| Activity: | Selenocysteine lyase, with EC number 4.4.1.16 | ||||||||
| Domains: | csdA, PRK09295 | ||||||||
| |||||||||
| |||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal Structure of selenocysteine lyase
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1JF9 is a 1 chain structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
- Lima CD. Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation. J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
Page seeded by OCA on Mon Feb 16 17:42:18 2009
Categories: Escherichia coli | Selenocysteine lyase | Burley, S K. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | Cysteine | New york structural genomix research consortium | Nif | Nysgxrc | Protein structure initiative | Psi | Pursulfide | Selenocysteine | Structural genomic
