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1iug
From Proteopedia
| 1iug, resolution 2.20Å () | |||||||||
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| Domains: | COG0075 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
The crystal structure of aspartate aminotransferase which belongs to subgroup IV from Thermus thermophilus
Protein TT0402 from Thermus thermophilus HB8 exhibits about 30-35% sequence identity with proteins belonging to subgroup IV in the aminotransferase family of the fold-type I pyridoxal 5'-phosphate (PLP)-dependent enzymes. In this study, we determined the crystal structure of TT0402 at 2.3 A resolution (R(factor) = 19.9%, R(free) = 23.6%). The overall structure of TT0402 exhibits the fold conserved in aminotransferases, and is most similar to that of the Escherichia coli phosphoserine aminotransferase, which belongs to subgroup IV but shares as little as 13% sequence identity with TT0402. Kinetic assays confirmed that TT0402 has higher transamination activities with the amino group donor, L-glutamate, and somewhat lower activities with L-aspartate. These results indicate that TT0402 is a subgroup IV aminotransferase for the synthesis/degradation of either L-aspartate or a similar compound.
Crystal structure of a putative aspartate aminotransferase belonging to subgroup IV., Katsura Y, Shirouzu M, Yamaguchi H, Ishitani R, Nureki O, Kuramitsu S, Hayashi H, Yokoyama S, Proteins. 2004 May 15;55(3):487-92. PMID:15103612
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1IUG is a 2 chains structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
- Katsura Y, Shirouzu M, Yamaguchi H, Ishitani R, Nureki O, Kuramitsu S, Hayashi H, Yokoyama S. Crystal structure of a putative aspartate aminotransferase belonging to subgroup IV. Proteins. 2004 May 15;55(3):487-92. PMID:15103612 doi:10.1002/prot.20020
Page seeded by OCA on Tue Feb 17 15:06:34 2009
Categories: Thermus thermophilus | Hayashi, H. | Ishitani, R. | Katsura, Y. | Kuramitsu, S. | Nureki, O. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shirouzu, M. | Yamaguchi, H. | Yokoyama, S. | Pyridoxal-5'-phosphate form | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Wild type
