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1iuc
From Proteopedia
| 1iuc, resolution 2.24Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sites: | , , and | ||||||||
| Ligands: | , , | ||||||||
| Related: | 1iub | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Fucose-specific lectin from Aleuria aurantia with three ligands
Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely used as a specific probe for fucose. Fucosylated sugars often play pivotal roles in many cellular processes. We have determined the crystal structure of AAL at 2.24 A resolution in complex with only three fucose molecules in its five sugar binding sites of a six-fold beta-propeller structure. Very recently, the structure of AAL has been independently determined, showing that all the five binding sites were occupied by fucose molecules [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]. Stabilization of the arginine conformation bound to fucose molecules plays an essential role in generating the difference in the affinity in the five binding sites. Binding models with a couple of saccharides based on biochemical assays suggest that hydrophobic contacts also play important roles in AAL recognizing its ligand.
Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites., Fujihashi M, Peapus DH, Kamiya N, Nagata Y, Miki K, Biochemistry. 2003 Sep 30;42(38):11093-9. PMID:14503859
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1IUC is a 1 chain structure of sequence from Aleuria aurantia. Full crystallographic information is available from OCA.
Reference
- Fujihashi M, Peapus DH, Kamiya N, Nagata Y, Miki K. Crystal structure of fucose-specific lectin from Aleuria aurantia binding ligands at three of its five sugar recognition sites. Biochemistry. 2003 Sep 30;42(38):11093-9. PMID:14503859 doi:10.1021/bi034983z
Page seeded by OCA on Thu Sep 3 16:21:41 2009
