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1gec

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1gec, resolution 2.10Å ()
Non-Standard Residues: ,
Activity: Glycyl endopeptidase, with EC number 3.4.22.25
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



GLYCYL ENDOPEPTIDASE-COMPLEX WITH BENZYLOXYCARBONYL-LEUCINE-VALINE-GLYCINE-METHYLENE COVALENTLY BOUND TO CYSTEINE 25

Publication Abstract from PubMed

Glycyl endopeptidase is a cysteine endopeptidase of the papain family, characterized by specificity for cleavage C-terminal to glycyl residues only and by resistance to inhibition by members of the cystatin family of cysteine proteinase inhibitors. Glycyl endopeptidase has been crystallized from high salt with a substrate-like inhibitor covalently bound to the catalytic Cys 25. The structure has been solved by molecular replacement with the structure of papain and refined at 2.1 A to an R factor of 0.196 (Rfree = 0.258) with good geometry. The structure of the S1 substrate binding site of glycyl endopeptidase differs from that of papain by the substitution of glycines at residues 23 and 65 in papain, with glutamic acid and arginine, respectively, in glycyl endopeptidase. The side chains of these residues form a barrier across the binding pocket, effectively excluding substrate residues with large side chains from the S1 subsite. The constriction of this subsite in glycyl endopeptidase explains the unique specificity of this enzyme for cleavage after glycyl residues and is a major component of its resistance to inhibition by cystatins.

Crystal structure of glycyl endopeptidase from Carica papaya: a cysteine endopeptidase of unusual substrate specificity., O'Hara BP, Hemmings AM, Buttle DJ, Pearl LH, Biochemistry. 1995 Oct 10;34(40):13190-5. PMID:7548082

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1gec is a 2 chain structure with sequence from Carica papaya. Full crystallographic information is available from OCA.

Reference

  • O'Hara BP, Hemmings AM, Buttle DJ, Pearl LH. Crystal structure of glycyl endopeptidase from Carica papaya: a cysteine endopeptidase of unusual substrate specificity. Biochemistry. 1995 Oct 10;34(40):13190-5. PMID:7548082

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