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1gcc
From Proteopedia
| 1gcc, 1 NMR models () | |||||||||
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| Gene: | ATERF1 (Arabidopsis thaliana) | ||||||||
| Domains: | AP2 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
SOLUTION NMR STRUCTURE OF THE COMPLEX OF GCC-BOX BINDING DOMAIN OF ATERF1 AND GCC-BOX DNA, MINIMIZED AVERAGE STRUCTURE
The 3D solution structure of the GCC-box binding domain of a protein from Arabidopsis thaliana in complex with its target DNA fragment has been determined by heteronuclear multidimensional NMR in combination with simulated annealing and restrained molecular dynamic calculation. The domain consists of a three-stranded anti-parallel beta-sheet and an alpha-helix packed approximately parallel to the beta-sheet. Arginine and tryptophan residues in the beta-sheet are identified to contact eight of the nine consecutive base pairs in the major groove, and at the same time bind to the sugar phosphate backbones. The target DNA bends slightly at the central CG step, thereby allowing the DNA to follow the curvature of the beta-sheet.
A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA., Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M, EMBO J. 1998 Sep 15;17(18):5484-96. PMID:9736626
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1GCC is a 3 chains structure of sequences from Arabidopsis thaliana. Full experimental information is available from OCA.
Reference
- Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M. A novel mode of DNA recognition by a beta-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J. 1998 Sep 15;17(18):5484-96. PMID:9736626 doi:10.1093/emboj/17.18.5484
Page seeded by OCA on Mon Feb 16 16:54:24 2009
