Structural highlights
Function
CDAS_BACSP Hydrolyzes alpha-, beta- and gamma-cyclodextrins and the resulting linear maltodextrins, with the highest activity with beta-cyclodextrin (cyclomaltoheptaose). Soluble starch is hydrolyzed slowly, but it is nevertheless preferred over pullulan as a substrate. Is able to hydrolyze amylose and amylopectin, with a very strong preference for amylose, with maltose and glucose as the main products (PubMed:9606956, PubMed:10620329, PubMed:16536613). Maltose and glucose are the main hydrolysis products of cyclomaltodextrins, maltodextrins and starch, whereas panose is the main hydrolysis product of pullulan. Acarbose is partially hydrolyzed to glucose and pseudotrisaccharide. No activity with maltose as substrate (PubMed:9606956, PubMed:10620329). Has transglycosylating activity with high concentrations of maltotriose, maltotetraose and starch (PubMed:9606956).[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Kim MJ, Park WS, Lee HS, Kim TJ, Shin JH, Yoo SH, Cheong TK, Ryu S, Kim JC, Kim JW, Moon TW, Robyt JF, Park KH. Kinetics and inhibition of cyclomaltodextrinase from alkalophilic Bacillus sp. I-5. Arch Biochem Biophys. 2000 Jan 1;373(1):110-5. PMID:10620329 doi:10.1006/abbi.1999.1471
- ↑ Auh JH, Chae HY, Kim YR, Shim KH, Yoo SH, Park KH. Modification of rice starch by selective degradation of amylose using alkalophilic Bacillus cyclomaltodextrinase. J Agric Food Chem. 2006 Mar 22;54(6):2314-9. PMID:16536613 doi:10.1021/jf051887r
- ↑ Kim TJ, Shin JH, Oh JH, Kim MJ, Lee SB, Ryu S, Kwon K, Kim JW, Choi EH, Robyt JF, Park KH. Analysis of the gene encoding cyclomaltodextrinase from alkalophilic Bacillus sp. I-5 and characterization of enzymatic properties. Arch Biochem Biophys. 1998 May 15;353(2):221-7. PMID:9606956 doi:10.1006/abbi.1998.0639
