1e8u

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1e8u, resolution 2.00Å ()
Ligands: , ,
Activity: Exo-alpha-sialidase, with EC number 3.2.1.18
Related: 1e8t, 1e8v
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

STRUCTURE OF THE MULTIFUNCTIONAL PARAMYXOVIRUS HEMAGGLUTININ-NEURAMINIDASE

Publication Abstract from PubMed

Paramyxoviruses are the main cause of respiratory disease in children. One of two viral surface glycoproteins, the hemagglutinin-neuraminidase (HN), has several functions in addition to being the major surface antigen that induces neutralizing antibodies. Here we present the crystal structures of Newcastle disease virus HN alone and in complex with either an inhibitor or with the beta-anomer of sialic acid. The inhibitor complex reveals a typical neuraminidase active site within a beta-propeller fold. Comparison of the structures of the two complexes reveal differences in the active site, suggesting that the catalytic site is activated by a conformational switch. This site may provide both sialic acid binding and hydrolysis functions since there is no evidence for a second sialic acid binding site in HN. Evidence for a single site with dual functions is examined and supported by mutagenesis studies. The structure provides the basis for the structure-based design of inhibitors for a range of paramyxovirus-induced diseases.

Crystal structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase., Crennell S, Takimoto T, Portner A, Taylor G, Nat Struct Biol. 2000 Nov;7(11):1068-74. PMID:11062565

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1e8u is a 2 chain structure with sequence from Newcastle disease virus. Full crystallographic information is available from OCA.

See Also

Reference

  • Crennell S, Takimoto T, Portner A, Taylor G. Crystal structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase. Nat Struct Biol. 2000 Nov;7(11):1068-74. PMID:11062565 doi:10.1038/81002
  • Takimoto T, Taylor GL, Crennell SJ, Scroggs RA, Portner A. Crystallization of Newcastle disease virus hemagglutinin-neuraminidase glycoprotein. Virology. 2000 Apr 25;270(1):208-14. PMID:10772993 doi:10.1006/viro.2000.0263

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