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1b1y

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1b1y, resolution 2.50Å ()
Ligands: ,
Activity: Beta-amylase, with EC number 3.2.1.2
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

SEVENFOLD MUTANT OF BARLEY BETA-AMYLASE

Publication Abstract from PubMed

The three-dimensional structure of the sevenfold mutant of barley beta-amylase (BBA-7s) with increased thermostability was determined by X-ray crystallography. The enzyme was purified as a single component and crystallized by a hanging drop method in the presence of 14 % PEG 6000. The crystals belong to space group P43212 with cell dimensions a=b=72.11 A, c=250.51 A. The diffraction data up to 2.5 A were collected after soaking the crystal in 100 mM maltose with Rsym of 8.6 %. The structure was determined by a molecular replacement method using soybean beta-amylase (SBA) as a search model and refined to an R-factor of 18.7 %. The final model included 500 amino acid residues, 141 water molecules and three glucose residues, which were located at subsites 1-2 and 4 in the active site. The r.m.s. distance of 485 Calpha atoms between BBA-7s and SBA was 0.62 A. Out of the seven mutated amino acids, four (Ser295Ala, Ile297Val, Ser351Pro and Ala376Ser) were substitutions from the common residues with SBA to the thermostable forms. A comparison of the structures of BBA-7s and SBA indicated that the side-chain of Ser376 makes new hydrogen bonds to the main-chain of an adjacent beta-strand, and that the side-chains of Val297 reduce an unfavorable interaction between the side-chains of Ala314. The mutation of Ser295Ala breaks the hydrogen bond between Ser295 OG and Tyr195 OH, which seems to be the reason for the unoccupied glucose residue at subsite 3. The tandem mutations at 350-352 including substitutions to two Pro residues suggested the reduction of main-chain entropy in the unfolded structure of this solvent-exposed protruded loop.

The crystal structure of the sevenfold mutant of barley beta-amylase with increased thermostability at 2.5 A resolution., Mikami B, Yoon HJ, Yoshigi N, J Mol Biol. 1999 Jan 22;285(3):1235-43. PMID:9918723

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

1b1y is a 1 chain structure with sequence from Hordeum vulgare. Full crystallographic information is available from OCA.

See Also

Reference

  • Mikami B, Yoon HJ, Yoshigi N. The crystal structure of the sevenfold mutant of barley beta-amylase with increased thermostability at 2.5 A resolution. J Mol Biol. 1999 Jan 22;285(3):1235-43. PMID:9918723 doi:10.1006/jmbi.1998.2379
  • Karthikeyan S, Yadav S, Paramasivam M, Srinivasan A, Singh TP. Structure of buffalo lactoferrin at 3.3 A resolution at 277 K. Acta Crystallogr D Biol Crystallogr. 2000 Jun;56(Pt 6):684-9. PMID:10818344

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