3pyt

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3pyt, resolution 3.40Å ()
Ligands:
Related: 3pys, 3pyu, 3pyv, 3pyn, 3pyo, 3pyq, 3pyr


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

Crystal structure of a complex containing domain 3 of CrPV IGR IRES RNA bound to the 70S ribosome. This file contains the 50S subunit of the first 70S ribosome.

Publication Abstract from PubMed

Internal ribosome entry site (IRES) RNAs are elements of viral or cellular mRNAs that bypass steps of canonical eukaryotic cap-dependent translation initiation. Understanding of the structural basis of IRES mechanisms is limited, partially due to a lack of high-resolution structures of IRES RNAs bound to their cellular targets. Prompted by the universal phylogenetic conservation of the ribosomal P site, we solved the crystal structures of proposed P site binding domains from two intergenic region IRES RNAs bound to bacterial 70S ribosomes. The structures show that these IRES domains nearly perfectly mimic a tRNA*mRNA interaction. However, there are clear differences in the global shape and position of this IRES domain in the intersubunit space compared to those of tRNA, supporting a mechanism for IRES action that invokes hybrid state mimicry to drive a noncanonical mode of translocation. These structures suggest how relatively small structured RNAs can manipulate complex biological machines.

Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome., Zhu J, Korostelev A, Costantino DA, Donohue JP, Noller HF, Kieft JS, Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1839-44. Epub 2011 Jan 18. PMID:21245352

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3pyt is a 31 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

See Also

Reference

  • Zhu J, Korostelev A, Costantino DA, Donohue JP, Noller HF, Kieft JS. Crystal structures of complexes containing domains from two viral internal ribosome entry site (IRES) RNAs bound to the 70S ribosome. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):1839-44. Epub 2011 Jan 18. PMID:21245352 doi:10.1073/pnas.1018582108

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