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3izu

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3izu, resolution 8.25Å ()
Non-Standard Residues: , , , , , , , , , , , , ,
Related: 3izw


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

Structural insights into cognate vs. near-cognate discrimination during decoding. This entry contains the large subunit of a ribosome programmed with a cognate codon

Publication Abstract from PubMed

The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near-cognate Trp-tRNA(Trp) in the presence of kirromycin. Going through more than 350 000 images and employing image classification procedures, we find approximately 8% in which the TC is bound to the ribosome. The reconstructed 3D map provides a means to characterize the arrangement of the near-cognate aa-tRNA with respect to elongation factor Tu (EF-Tu) and the ribosome, as well as the domain movements of the ribosome. One of the interesting findings is that near-cognate tRNA's acceptor stem region is flexible and CCA end becomes disordered. The data bring direct structural insights into the induced-fit mechanism of decoding by the ribosome, as the analysis of the interactions between small and large ribosomal subunit, aa-tRNA and EF-Tu and comparison with the cognate case (UGG codon) offers clues on how the conformational signals conveyed to the GTPase differ in the two cases.

Structural insights into cognate versus near-cognate discrimination during decoding., Agirrezabala X, Schreiner E, Trabuco LG, Lei J, Ortiz-Meoz RF, Schulten K, Green R, Frank J, EMBO J. 2011 Mar 4. PMID:21378755

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3izu is a 33 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

See Also

Reference

  • Agirrezabala X, Schreiner E, Trabuco LG, Lei J, Ortiz-Meoz RF, Schulten K, Green R, Frank J. Structural insights into cognate versus near-cognate discrimination during decoding. EMBO J. 2011 Mar 4. PMID:21378755 doi:10.1038/emboj.2011.58

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