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3izs

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3izs, resolution 8.80Å ()
Non-Standard Residues:
Related: 3iz5, 3iz6, 3iz7, 3iz9, 3izb, 3izd, 3ize, 3izf


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Localization of the large subunit ribosomal proteins into a 6.1 A cryo-EM map of Saccharomyces cerevisiae translating 80S ribosome

Publication Abstract from PubMed

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-A resolution. This map, together with a 6.1-A map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model approximately 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution., Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R, Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19748-53. Epub 2010 Oct 27. PMID:20980660

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3izs is a 51 chain structure with sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 3izc. Full crystallographic information is available from OCA.

See Also

Reference

  • Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R. Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19748-53. Epub 2010 Oct 27. PMID:20980660 doi:10.1073/pnas.1009999107
  • Armache JP, Jarasch A, Anger AM, Villa E, Becker T, Bhushan S, Jossinet F, Habeck M, Dindar G, Franckenberg S, Marquez V, Mielke T, Thomm M, Berninghausen O, Beatrix B, Soding J, Westhof E, Wilson DN, Beckmann R. Localization of eukaryote-specific ribosomal proteins in a 5.5-A cryo-EM map of the 80S eukaryotic ribosome. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19754-9. Epub 2010 Oct 25. PMID:20974910 doi:10.1073/pnas.1010005107

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