3f1f

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3f1f, resolution 3.00Å ()
Ligands:
Related: 3f1e, 3f1g, 3f1h, 3d5a, 3d5b, 3d5c, 3d5d
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

Crystal structure of a translation termination complex formed with release factor RF2. This file contains the 50S subunit of one 70S ribosome. The entire crystal structure contains two 70S ribosomes as described in remark 400.

Publication Abstract from PubMed

We report the crystal structure of a translation termination complex formed by the Thermus thermophilus 70S ribosome bound with release factor RF2, in response to a UAA stop codon, solved at 3 A resolution. The backbone of helix alpha5 and the side chain of serine of the conserved SPF motif of RF2 recognize U1 and A2 of the stop codon, respectively. A3 is unstacked from the first 2 bases, contacting Thr-216 and Val-203 of RF2 and stacking on G530 of 16S rRNA. The structure of the RF2 complex supports our previous proposal that conformational changes in the ribosome in response to recognition of the stop codon stabilize rearrangement of the switch loop of the release factor, resulting in docking of the universally conserved GGQ motif in the PTC of the 50S subunit. As seen for the RF1 complex, the main-chain amide nitrogen of glutamine in the GGQ motif is positioned to contribute directly to catalysis of peptidyl-tRNA hydrolysis, consistent with mutational studies, which show that most side-chain substitutions of the conserved glutamine have little effect. We show that when the H-bonding capability of the main-chain N-H of the conserved glutamine is eliminated by substitution with proline, peptidyl-tRNA esterase activity is abolished, consistent with its proposed role in catalysis.

Crystal structure of a translation termination complex formed with release factor RF2., Korostelev A, Asahara H, Lancaster L, Laurberg M, Hirschi A, Zhu J, Trakhanov S, Scott WG, Noller HF, Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19684-9. Epub 2008 Dec 8. PMID:19064930

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3f1f is a 30 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

See Also

Reference

  • Korostelev A, Asahara H, Lancaster L, Laurberg M, Hirschi A, Zhu J, Trakhanov S, Scott WG, Noller HF. Crystal structure of a translation termination complex formed with release factor RF2. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19684-9. Epub 2008 Dec 8. PMID:19064930 doi:10.1073/pnas.0810953105

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