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2i2v
From Proteopedia
| 2i2v, resolution 3.22Å () | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , and | ||||||||
| Ligands: | , | ||||||||
| Related: | 2i2p, 2i2t, 2i2u | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 50s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.
Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
Structural basis for mRNA and tRNA positioning on the ribosome., Berk V, Zhang W, Pai RD, Cate JH, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2i2v is a 30 chain structure of Ribosomal protein L2, Ribosomal protein L3, Ribosomal protein L4, Ribosomal protein L5, Ribosomal protein L6, Ribosomal protein L9 and Ribosome with sequence from Escherichia coli. Full crystallographic information is available from OCA.
See Also
- Large Ribosomal Subunit of Haloarcula
- Ribosomal protein L2
- Ribosomal protein L3
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L9
- Ribosome
- User:Wayne Decatur/Haloarcula Large Ribosomal Subunit
Reference
- Berk V, Zhang W, Pai RD, Cate JH. Structural basis for mRNA and tRNA positioning on the ribosome. Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497
