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2r1x
From Proteopedia
| 2r1x, resolution 1.60Å () | |||||||||
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| Ligands: | , , , | ||||||||
| Related: | 1q9q, 2r1w, 2r1y, 2r23, 2r2b, 2r2e, 2r2h
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of S25-2 Fab in complex with Kdo analogues
To explore the molecular basis of antigen recognition by germline antibodies, we have determined to high resolution the structures of the near-germline monoclonal antibody S25-2 in complex with seven distinct carbohydrate antigens based on the bacterial sugar 3-deoxy-alpha-D-manno-oct-2-ulosonic acid (Kdo). In contrast to previous findings, the inherited germline Kdo monosaccharide binding site is not restricted to this bacterial sugar but is able to accommodate an array of substitutions and chemical modifications of Kdo, including naturally occurring antigens containing the related monosaccharide d-glycero-alpha-d-talo-oct-2-ulosonic acid as well as nonterminal Kdo residues. However, we show by surface plasmon resonance and ELISA how antibody S25-2 specificity is so dependent on the context in which the antigen is presented that a free disaccharide displays strong binding while the same lipid-A-bound disaccharide does not bind. These structures provide insight into how inherited germline genes code for immunoglobulins of limited flexibility that are capable of binding a range of epitopes from which affinity-matured antibodies are generated.
Exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes., Brooks CL, Muller-Loennies S, Brade L, Kosma P, Hirama T, MacKenzie CR, Brade H, Evans SV, J Mol Biol. 2008 Mar 21;377(2):450-68. Epub 2008 Jan 16. PMID:18272175
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2R1X is a 2 chains structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes., Brooks CL, Muller-Loennies S, Brade L, Kosma P, Hirama T, MacKenzie CR, Brade H, Evans SV, J Mol Biol. 2008 Mar 21;377(2):450-68. Epub 2008 Jan 16. PMID:18272175
Page seeded by OCA on Wed Dec 31 10:07:37 2008
