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WspR

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Overview

WspR 3bre

from Pseudomonas aeruginosa is a response regulator with an catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver domain () and a C-terminal domain that confers the catalytic activity with all canonical present.



Although not modified (i.e. phosphorylated) at the active Asp (), the Rec domains mediate formation of WspR. Two dimers, in turn, are associated by head-to-head contact to a of approximate 222 (D2) symmetry.












Allosteric product binding site

WspR 3bre

There are two allosteric sites ( and ) that become cross-linked by (c-di-GMP)2 dimers in the molecule. For a close-up click (, , ). Note that there are four (c-di-GMP)2 dimers per WspR tetramer.






3D structures of response regulator

Response regulator

References

WspR structure 3bre:

  • De N, Pirruccello M, Krasteva PV, Bae N, Raghavan RV, Sondermann H. Phosphorylation-independent regulation of the diguanylate cyclase WspR. PLoS Biol. 2008 Mar 25;6(3):e67. PMID:18366254 doi:10.1371/journal.pbio.0060067

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Michal Harel, Tilman Schirmer

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