Molecular Playground/ClpP

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Here, in the Chien lab of the University of Massachusetts-Amherst, we study how proteolysis plays a large part in protein quality control. The maintenance and timely destruction of protein levels plays an important role during cell homeostasis and cell transitions/differentiation, yet much of what governs these processes has to be fully understood.


E. coli ClpP protease complex with MPD (PDB code 1yg6).

Drag the structure with the mouse to rotate

References

1. ClpP: A structurally dynamic protease regulated by AAA+ proteins. Alexopoulos JA et. al (2012 J Struct Bio)

2. ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes. Ortega J et. al (2004 J. Struct Biol.)

3. Binding of the ClpA Unfoldase Opens the Axial Gate of ClpP Peptidase. Effantin et. al (2010 J Biol Chem)

4. Turned on for degradation: ATPase-independent degradation by ClpP. Bewley MC et. al (2009 J Struct Biol)

5. Control of peptide product sizes by the energy-dependent protease ClpAP. Choi KH et. al (2005 Biochemistry)

6. Clp P represents a unique family of serine proteases. Maurizi MR et. al (1990 J Biolchem)

7. ClpP: a distinctive family of cylindrical energy-dependent serine proteases. Yu AY et. al (2007 FEBS Lett.)

8. Human mitochondrial ClpP is a stable heptamer that assembles into a tetradecamer in the presence of ClpX. Kang SG et. al (2007 J Biol Chem)

9. The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. Gottesman S et. al (1998 Genes Dev.)

10. Bacterial Cell Stress Protein ClpP: A Novel Antibiotic Target. Brötz-Oesterhelt et. al (2013 Heat Shock Proteins Volume 7, pp 375-385)

11. Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26 S proteasome. Kessel M et. al (1995 J Mol Bio)

12. Proteasomes and their kin: proteases in the machine age. Pickart CM et. al (2004 Nat Rev Mol Cell Biol.)


Acknowledgements

Kamal Joshi, Joanne Lau, Jing Liu, Rob Vass, Lisa Hernandez

PDB id:1YG6 from Bewley, MC et. al (2006 J.Struct.Biol.)

Proteopedia Page Contributors and Editors (what is this?)

Robert Vass, Lisa Hernandez, Michal Harel

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