Structural highlights
Function
RHLA_PSEAE Required for rhamnolipid surfactant production (PubMed:15126453). Supplies the acyl moieties for rhamnolipid biosynthesis by competing with the enzymes of the type II fatty acid synthase (FASII) cycle for the beta-hydroxyacyl-acyl carrier protein (ACP) pathway intermediates. Catalyzes the formation of one molecule of beta-hydroxydecanoyl-beta-hydroxydecanoate from two molecules of beta-hydroxydecanoyl-ACP. Is the only enzyme required to generate the lipid component of rhamnolipid. In vitro results establish that RhlA is highly selective for 10-carbon acyl-ACP intermediates and thus functions as the molecular ruler that controls the acyl chain composition of rhamnolipids. Cannot use beta-hydroxydecanoyl-CoA as substrate (PubMed:18326581). Rhamnolipid production plays an important role in swarming motility (PubMed:15126453).[1] [2]
References
- ↑ Heurlier K, Williams F, Heeb S, Dormond C, Pessi G, Singer D, Cámara M, Williams P, Haas D. Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1. J Bacteriol. 2004 May;186(10):2936-45. PMID:15126453 doi:10.1128/JB.186.10.2936-2945.2004
- ↑ Zhu K, Rock CO. RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa. J Bacteriol. 2008 May;190(9):3147-54. PMID:18326581 doi:10.1128/JB.00080-08
