Structural highlights
Publication Abstract from PubMed
Biological activity is governed by the timely redistribution of molecular interactions, and static structural snapshots often appear insufficient to provide the molecular determinants that choreograph communication. This conundrum applies to multidomain enzymatic systems called nonribosomal peptide synthetases (NRPSs), which assemble simple substrates into complex metabolites, where a dynamic domain organization challenges rational design to produce new pharmaceuticals. Using a nuclear magnetic resonance (NMR) atomic-level readout of biochemical transformations, we demonstrate that global structural fluctuations help promote substrate-dependent communication and allosteric responses, and impeding these global dynamics by a point-site mutation hampers allostery and molecular recognition. Our results establish global structural dynamics as sensors of molecular events that can remodel domain interactions, and they provide new perspectives on mechanisms of allostery, protein communication, and NRPS synthesis.
Global protein dynamics as communication sensors in peptide synthetase domains.,Mishra SH, Kancherla AK, Marincin KA, Bouvignies G, Nerli S, Sgourakis N, Dowling DP, Frueh DP Sci Adv. 2022 Jul 15;8(28):eabn6549. doi: 10.1126/sciadv.abn6549. Epub 2022 Jul, 15. PMID:35857508[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mishra SH, Kancherla AK, Marincin KA, Bouvignies G, Nerli S, Sgourakis N, Dowling DP, Frueh DP. Global protein dynamics as communication sensors in peptide synthetase domains. Sci Adv. 2022 Jul 15;8(28):eabn6549. doi: 10.1126/sciadv.abn6549. Epub 2022 Jul, 15. PMID:35857508 doi:http://dx.doi.org/10.1126/sciadv.abn6549
