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Publication Abstract from PubMed

Efficient incorporation of modified nucleotides by DNA polymerases is essential for many cutting-edge biomolecular technologies. The present study compares the acceptance of either alkene- or alkyne-modified nucleotides by KlenTaq DNA polymerase and provides structural insights into how 7-deaza-adenosine and thymidine with attached alkene-modifications are incorporated into the growing DNA strand. Thereby, we identified modified nucleotides, which prove to be superior substrates for KlenTaq DNA polymerase compared to their natural analogues. The knowledge can be used to guide future design of functionalized nucleotide building blocks.

Structural basis for the KlenTaq DNA Polymerase catalyzed Incorporation of Alkene- versus Alkyne-modified Nucleotides.,Marx A, Hottin A, Betz K, Diederichs K Chemistry. 2016 Nov 30. doi: 10.1002/chem.201604515. PMID:27901305^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.