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5bca

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5bca, resolution 2.20Å ()
Ligands:
Activity: Beta-amylase, with EC number 3.2.1.2
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

BETA-AMYLASE FROM BACILLUS CEREUS VAR. MYCOIDES

Publication Abstract from PubMed

The crystal structure of beta-amylase from Bacillus cereus var. mycoides was determined by the multiple isomorphous replacement method. The structure was refined to a final R-factor of 0.186 for 102,807 independent reflections with F/sigma(F) > or = 2.0 at 2.2 A resolution with root-mean-square deviations from ideality in bond lengths, and bond angles of 0.014 A and 3.00 degrees, respectively. The asymmetric unit comprises four molecules exhibiting a dimer-of-dimers structure. The enzyme, however, acts as a monomer in solution. The beta-amylase molecule folds into three domains; the first one is the N-terminal catalytic domain with a (beta/alpha)8 barrel, the second one is the excursion part from the first one, and the third one is the C-terminal domain with two almost anti-parallel beta-sheets. The active site cleft, including two putative catalytic residues (Glu172 and Glu367), is located on the carboxyl side of the central beta-sheet in the (beta/alpha)8 barrel, as in most amylases. The active site structure of the enzyme resembles that of soybean beta-amylase with slight differences. One calcium ion is bound per molecule far from the active site. The C-terminal domain has a fold similar to the raw starch binding domains of cyclodextrin glycosyltransferase and glucoamylase.

Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution., Oyama T, Kusunoki M, Kishimoto Y, Takasaki Y, Nitta Y, J Biochem. 1999 Jun;125(6):1120-30. PMID:10348915

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

5bca is a 4 chain structure with sequence from Bacillus cereus. Full crystallographic information is available from OCA.

See Also

Reference

  • Oyama T, Kusunoki M, Kishimoto Y, Takasaki Y, Nitta Y. Crystal structure of beta-amylase from Bacillus cereus var. mycoides at 2.2 A resolution. J Biochem. 1999 Jun;125(6):1120-30. PMID:10348915

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