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|4rub, resolution 2.70Å ()|
A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE
A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 220.127.116.11) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.
A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state., Suh SW, Cascio D, Chapman MS, Eisenberg D, J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Suh SW, Cascio D, Chapman MS, Eisenberg D. A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state. J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999