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4rhv

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4rhv, resolution 3.00Å ()
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THE USE OF MOLECULAR-REPLACEMENT PHASES FOR THE REFINEMENT OF THE HUMAN RHINOVIRUS 14 STRUCTURE

Publication Abstract from PubMed

The structure of human rhinovirus 14 has been refined, by the method of restrained least squares, to an R factor of 0.16 for various random samples between 6 and 3 A resolution with F greater than 3 sigma (F). As a first step the non-crystallographic symmetry parameters were optimized using the initial atomic model in a rigid-body refinement procedure. Phase determination by the molecular-replacement phase extension and refinement procedure was continued to 2.94 A resolution, employing the improved non-crystallographic symmetry operators. The resultant structure-factor phases and weights, together with the measured amplitudes, constituted the X-ray observations used in the restrained refinement. The Hendrickson-Konnert program system [Konnert & Hendrickson (1980). Acta Cryst. A36, 344-350] was modified to incorporate non-crystallographic symmetry constrains and structure-factor phases as observations. The non-bonded contacts between subunits related by non-crystallographic symmetry were also restrained.

The use of molecular-replacement phases for the refinement of the human rhinovirus 14 structure., Arnold E, Rossmann MG, Acta Crystallogr A. 1988 May 1;44 ( Pt 3):270-82. PMID:2856083

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

4RHV is a 4 chains structure with sequences from Human rhinovirus 14. This structure supersedes the now removed PDB entries and 1rhv. The August 2001 RCSB PDB Molecule of the Month feature on Poliovirus and Rhinovirus by David S. Goodsell is 10.2210/rcsb_pdb/mom_2001_8. Full crystallographic information is available from OCA.

Reference

  • Arnold E, Rossmann MG. The use of molecular-replacement phases for the refinement of the human rhinovirus 14 structure. Acta Crystallogr A. 1988 May 1;44 ( Pt 3):270-82. PMID:2856083

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