471d

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471d, resolution 2.70Å ()
Ligands:
Non-Standard Residues: , , ,
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA

Publication Abstract from PubMed

2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.

Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

471d is a 2 chain structure. Full crystallographic information is available from OCA.

Reference

  • Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M. Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA. Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355 doi:10.1038/9304

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