First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.

3tw5

From Proteopedia

Jump to: navigation, search


3tw5, resolution 2.95Å ()
Sites: and
Ligands:
Activity: Protein-glutamine gamma-glutamyltransferase, with EC number 2.3.2.13
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the GP42 transglutaminase from Phytophthora sojae

Publication Abstract from PubMed

Transglutaminases (TGases) are ubiquitous enzymes that catalyze selective crosslinking between protein-bound glutamine and lysine residues; the resulting isopeptide bond confers high resistance to proteolysis. Phytophthora sojae, a pathogen of soybean, secretes a Ca(2+)-dependent TGase (GP42) that is activating defense responses in both host and non-host plants. A GP42 fragment of 13 amino acids, termed Pep-13, was shown to be absolutely indispensable for both TGase and elicitor activity. GP42 does not share significant primary sequence similarity with known TGases from mammals or bacteria. This suggests that GP42 has evolved novel structural and catalytic features to support enzymatic activity. We have solved the crystal structure of the catalytically inactive point mutant GP42 (C290S) at 2.95 A resolution and identified residues involved in catalysis by mutational analysis. The protein comprises three domains that assemble into an elongated structure. Although GP42 has no structural homolog, its core region displays significant similarity to the catalytic core of the Mac-1 cysteine protease from Group A Streptococcus, a member of the papain-like superfamily of cysteine proteases. Proteins that are taxonomically related to GP42 are only present in plant pathogenic oomycetes belonging to the order of the Peronosporales (e.g Phytophthora, Hyaloperonospora and Pythium spp) and in marine Vibrio bacteria. This suggests that a lateral gene transfer event may have occurred between bacteria and oomycetes. Our results offer a basis to design and use highly specific inhibitors of the GP42-like TGase family that may impair the growth of important oomycete and bacterial pathogens.

Structural and phylogenetic analyses of the GP42 transglutaminase from Phytophthora sojae reveal an evolutionary relationship between oomycetes and marine Vibrio bacteria., Reiss K, Kirchner E, Gijzen M, Zocher G, Loeffelhardt B, Nuernberger T, Stehle T, Brunner F, J Biol Chem. 2011 Oct 12. PMID:21994936

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3tw5 is a 2 chain structure with sequence from Phytophthora sojae. Full crystallographic information is available from OCA.

Reference

  • Reiss K, Kirchner E, Gijzen M, Zocher G, Loeffelhardt B, Nuernberger T, Stehle T, Brunner F. Structural and phylogenetic analyses of the GP42 transglutaminase from Phytophthora sojae reveal an evolutionary relationship between oomycetes and marine Vibrio bacteria. J Biol Chem. 2011 Oct 12. PMID:21994936 doi:10.1074/jbc.M111.290544

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools