3t1e

From Proteopedia

Jump to: navigation, search
3t1e, resolution 3.30Å ()
Gene: HN (Newcastle disease virus)
Activity: Exo-alpha-sialidase, with EC number 3.2.1.18


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Contents

The structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a 4-helix bundle stalk

Publication Abstract from PubMed

The paramyxovirus hemagglutinin-neuraminidase (HN) protein plays multiple roles in viral entry and egress, including binding to sialic acid receptors, activating the fusion (F) protein to activate membrane fusion and viral entry, and cleaving sialic acid from carbohydrate chains. HN is an oligomeric integral membrane protein consisting of an N-terminal transmembrane domain, a stalk region, and an enzymatically active neuraminidase (NA) domain. Structures of the HN NA domains have been solved previously; however, the structure of the stalk region has remained elusive. The stalk region contains specificity determinants for F interactions and activation, underlying the requirement for homotypic F and HN interactions in viral entry. Mutations of the Newcastle disease virus HN stalk region have been shown to affect both F activation and NA activities, but a structural basis for understanding these dual affects on HN functions has been lacking. Here, we report the structure of the Newcastle disease virus HN ectodomain, revealing dimers of NA domain dimers flanking the N-terminal stalk domain. The stalk forms a parallel tetrameric coiled-coil bundle (4HB) that allows classification of extensive mutational data, providing insight into the functional roles of the stalk region. Mutations that affect both F activation and NA activities map predominantly to the 4HB hydrophobic core, whereas mutations that affect only F-protein activation map primarily to the 4HB surface. Two of four NA domains interact with the 4HB stalk, and residues at this interface in both the stalk and NA domain have been implicated in HN function.

Structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk., Yuan P, Swanson KA, Leser GP, Paterson RG, Lamb RA, Jardetzky TS, Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14920-5. Epub 2011 Aug 22. PMID:21873198

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3t1e is a 4 chain structure with sequence from Newcastle disease virus. Full crystallographic information is available from OCA.

See Also

Reference

  • Yuan P, Swanson KA, Leser GP, Paterson RG, Lamb RA, Jardetzky TS. Structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk. Proc Natl Acad Sci U S A. 2011 Sep 6;108(36):14920-5. Epub 2011 Aug 22. PMID:21873198 doi:10.1073/pnas.1111691108

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools