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3rgp, resolution 1.88Å ()
Ligands: ,
Activity: Catalase, with EC number
Related: 3re8, 3rgs

Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml


Structural and Kinetic Analysis of the Beef liver Catalase complexed with Nitric Oxide

Publication Abstract from PubMed

We present the structures of bovine catalase in its native form and complexed with ammonia and nitric oxide, obtained by X-ray crystallography. Using the NO generator 1-(N,N-diethylamino)diazen-1-ium-1,2-diolate, we were able to generate sufficiently high NO concentrations within the catalase crystals that substantial occupation was observed despite a high dissociation rate. Nitric oxide seems to be slightly bent from the heme normal that may indicate some iron(II) character in the formally ferric catalase. Microspectrophotometric investigations inline with the synchrotron X-ray beam reveal photoreduction of the central heme iron. In the cases of the native and ammonia-complexed catalase, reduction is accompanied by a relaxation phase. This is likely not the case for the catalase NO complex. The kinetics of binding of NO to catalase were investigated using NO photolyzed from N,N'-bis(carboxymethyl)-N,N'-dinitroso-p-phenylenediamine using an assay that combines catalase with myoglobin binding kinetics. The off rate is 1.5 s(-1). Implications for catalase function are discussed.

Interaction of Nitric Oxide with Catalase: Structural and Kinetic Analysis., Purwar N, McGarry JM, Kostera J, Pacheco AA, Schmidt M, Biochemistry. 2011 May 6. PMID:21524057

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3rgp is a 4 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA.

See Also


  • Purwar N, McGarry JM, Kostera J, Pacheco AA, Schmidt M. Interaction of Nitric Oxide with Catalase: Structural and Kinetic Analysis. Biochemistry. 2011 May 6. PMID:21524057 doi:10.1021/bi200130r

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