Alix and cellular paralogs HD-PTP and Brox contain N-terminal Bro1 domains that bind ESCRT-III CHMP4. In contrast to HD-PTP and Brox, expression of the Bro1 domain of Alix alleviates HIV-1 release defects as a result of interrupted access to ESCRT. In an attempt to elucidate this functional discrepancy, we solved the crystal structures of the Bro1 domains of HD-PTP and Brox. They revealed typical "boomerang" folds they share with the Bro1 Alix domain. However, they each contain unique structural features that may be relevant to their specific function(s). In particular, phenylalanine residue in position 105 (Phe105) of Alix belongs to a long loop that is unique to its Bro1 domain. Concurrently, mutation of Phe105 and surrounding residues at the tip of the loop compromise the function of Alix in HIV-1 budding without affecting its interactions with Gag or CHMP4. These studies identify a functional determinant in the Bro1 domain of Alix.
The Phe105 Loop of Alix Bro1 Domain Plays a Key Role in HIV-1 Release.,Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, Xiao TS, Bouamr F Structure. 2011 Sep 1. PMID:21889351
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P, Xiao TS, Bouamr F. The Phe105 Loop of Alix Bro1 Domain Plays a Key Role in HIV-1 Release. Structure. 2011 Sep 1. PMID:21889351 doi:10.1016/j.str.2011.07.016