3prd

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3prd, resolution 3.30Å ()
Gene: MJ0825 (Methanocaldococcus jannaschii)
Activity: Peptidylprolyl isomerase, with EC number 5.2.1.8
Related: 3pr9, 3pra, 3prb


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Structural analysis of protein folding by the Methanococcus jannaschii chaperone FKBP26

Publication Abstract from PubMed

In the cell, protein folding is mediated by folding catalysts and chaperones. The two functions are often linked, especially when the catalytic module forms part of a multidomain protein, as in Methanococcus jannaschii peptidyl-prolyl cis/trans isomerase FKBP26. Here, we show that FKBP26 chaperone activity requires both a 50-residue insertion in the catalytic FKBP domain, also called 'Insert-in-Flap' or IF domain, and an 80-residue C-terminal domain. We determined FKBP26 structures from four crystal forms and analyzed chaperone domains in light of their ability to mediate protein-protein interactions. FKBP26 is a crescent-shaped homodimer. We reason that folding proteins are bound inside the large crescent cleft, thus enabling their access to inward-facing peptidyl-prolyl cis/trans isomerase catalytic sites and ipsilateral chaperone domain surfaces. As these chaperone surfaces participate extensively in crystal lattice contacts, we speculate that the observed lattice contacts reflect a proclivity for protein associations and represent substrate interactions by FKBP26 chaperone domains. Finally, we find that FKBP26 is an exceptionally flexible molecule, suggesting a mechanism for nonspecific substrate recognition.

Structural Analysis of Protein Folding by the Long-Chain Archaeal Chaperone FKBP26., Martinez-Hackert E, Hendrickson WA, J Mol Biol. 2011 Apr 1;407(3):450-64. Epub 2011 Jan 22. PMID:21262232

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3prd is a 1 chain structure with sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

See Also

Reference

  • Martinez-Hackert E, Hendrickson WA. Structural Analysis of Protein Folding by the Long-Chain Archaeal Chaperone FKBP26. J Mol Biol. 2011 Apr 1;407(3):450-64. Epub 2011 Jan 22. PMID:21262232 doi:10.1016/j.jmb.2011.01.027

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