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3o8o

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3o8o, resolution 2.90Å ()
Ligands: ,
Gene: PFK1, YGR240C, G8599 (Saccharomyces cerevisiae), PFK2, YMR205C, YM8325.06C (Saccharomyces cerevisiae)
Activity: 6-phosphofructokinase, with EC number 2.7.1.11


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Structure of phosphofructokinase from Saccharomyces cerevisiae

Publication Abstract from PubMed

Phosphofructokinase 1 (PFK) is a multisubunit allosteric enzyme that catalyzes the principal regulatory step in glycolysis-the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate by ATP. The activity of eukaryotic PFK is modulated by a number of effectors in response to the cell's needs for energy and building blocks for biosynthesis. The crystal structures of eukaryotic PFKs-from Saccharomyces cerevisiae and rabbit skeletal muscle-demonstrate how successive gene duplications and fusion are reflected in the protein structure and how they allow the evolution of new functionalities. The basic framework inherited from prokaryotes is conserved, and additional levels of structural and functional complexity have evolved around it. Analysis of protein-ligand complexes has shown how PFK is activated by fructose 2,6-bisphosphate (a powerful PFK effector found only in eukaryotes) and reveals a novel nucleotide binding site. Crystallographic results have been used as the basis for structure-based effector design.

The Crystal Structures of Eukaryotic Phosphofructokinases from Baker's Yeast and Rabbit Skeletal Muscle., Banaszak K, Mechin I, Obmolova G, Oldham M, Chang SH, Ruiz T, Radermacher M, Kopperschlager G, Rypniewski W, J Mol Biol. 2011 Jan 15. PMID:21241708

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3o8o is a 8 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

See Also

Reference

  • Banaszak K, Mechin I, Obmolova G, Oldham M, Chang SH, Ruiz T, Radermacher M, Kopperschlager G, Rypniewski W. The Crystal Structures of Eukaryotic Phosphofructokinases from Baker's Yeast and Rabbit Skeletal Muscle. J Mol Biol. 2011 Jan 15. PMID:21241708 doi:10.1016/j.jmb.2011.01.019

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