3m9c

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3m9c, resolution 3.90Å ()
Non-Standard Residues:
Activity: NADH dehydrogenase (ubiquinone), with EC number 1.6.5.3
Related: 3m9s


Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


Crystal structure of the membrane domain of respiratory complex I from Escherichia coli

Publication Abstract from PubMed

Complex I is the first enzyme of the respiratory chain and has a central role in cellular energy production, coupling electron transfer between NADH and quinone to proton translocation by an unknown mechanism. Dysfunction of complex I has been implicated in many human neurodegenerative diseases. We have determined the structure of its hydrophilic domain previously. Here, we report the alpha-helical structure of the membrane domain of complex I from Escherichia coli at 3.9 A resolution. The antiporter-like subunits NuoL/M/N each contain 14 conserved transmembrane (TM) helices. Two of them are discontinuous, as in some transporters. Unexpectedly, subunit NuoL also contains a 110-A long amphipathic alpha-helix, spanning almost the entire length of the domain. Furthermore, we have determined the structure of the entire complex I from Thermus thermophilus at 4.5 A resolution. The L-shaped assembly consists of the alpha-helical model for the membrane domain, with 63 TM helices, and the known structure of the hydrophilic domain. The architecture of the complex provides strong clues about the coupling mechanism: the conformational changes at the interface of the two main domains may drive the long amphipathic alpha-helix of NuoL in a piston-like motion, tilting nearby discontinuous TM helices, resulting in proton translocation.

The architecture of respiratory complex I., Efremov RG, Baradaran R, Sazanov LA, Nature. 2010 May 27;465(7297):441-5. PMID:20505720

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3m9c is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

  • Efremov RG, Baradaran R, Sazanov LA. The architecture of respiratory complex I. Nature. 2010 May 27;465(7297):441-5. PMID:20505720 doi:10.1038/nature09066

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