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|3k7a, resolution 3.80Å ()|
|Gene:||D2150, RPB1, RPB220, RPO21, SUA8, YDL140C (Saccharomyces cerevisiae), RPB12, RPC10, YHR143BW, YHR143W-A (Saccharomyces cerevisiae), P9513.4, SUA7, YPR086W (Saccharomyces cerevisiae), RPB150, RPB2, RPO22, YOR151C (Saccharomyces cerevisiae), RPB3, YIL021W (Saccharomyces cerevisiae), RPA7, RPB5, RPC9, YBR1204, YBR154C (Saccharomyces cerevisiae), P9677.8, RPB6, RPO26, YPR187W (Saccharomyces cerevisiae), RPB8, YOR224C, YOR50-14 (Saccharomyces cerevisiae), RPB9, YGL070C (Saccharomyces cerevisiae), RPB10, YOR210W (Saccharomyces cerevisiae), RPB11, YOL005C (Saccharomyces cerevisiae)|
Crystal Structure of an RNA polymerase II-TFIIB complex
Previous x-ray crystal structures have given insight into the mechanism of transcription and the role of general transcription factors in the initiation of the process. A structure of an RNA polymerase II-general transcription factor TFIIB complex at 4.5 angstrom resolution revealed the amino-terminal region of TFIIB, including a loop termed the "B finger," reaching into the active center of the polymerase where it may interact with both DNA and RNA, but this structure showed little of the carboxyl-terminal region. A new crystal structure of the same complex at 3.8 angstrom resolution obtained under different solution conditions is complementary with the previous one, revealing the carboxyl-terminal region of TFIIB, located above the polymerase active center cleft, but showing none of the B finger. In the new structure, the linker between the amino- and carboxyl-terminal regions can also be seen, snaking down from above the cleft toward the active center. The two structures, taken together with others previously obtained, dispel long-standing mysteries of the transcription initiation process.
Structure of an RNA polymerase II-TFIIB complex and the transcription initiation mechanism., Liu X, Bushnell DA, Wang D, Calero G, Kornberg RD, Science. 2010 Jan 8;327(5962):206-9. Epub 2009 Nov 12. PMID:19965383
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.