|3iyn, resolution 3.60Å ()|
3.6-Angstrom cryoEM structure of human adenovirus type 5
Construction of a complex virus may involve a hierarchy of assembly elements. Here, we report the structure of the whole human adenovirus virion at 3.6 angstroms resolution by cryo-electron microscopy (cryo-EM), revealing in situ atomic models of three minor capsid proteins (IIIa, VIII, and IX), extensions of the (penton base and hexon) major capsid proteins, and interactions within three protein-protein networks. One network is mediated by protein IIIa at the vertices, within group-of-six (GOS) tiles--a penton base and its five surrounding hexons. Another is mediated by ropes (protein IX) that lash hexons together to form group-of-nine (GON) tiles and bind GONs to GONs. The third, mediated by IIIa and VIII, binds each GOS to five surrounding GONs. Optimization of adenovirus for cancer and gene therapy could target these networks.
Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks., Liu H, Jin L, Koh SB, Atanasov I, Schein S, Wu L, Zhou ZH, Science. 2010 Aug 27;329(5995):1038-43. PMID:20798312
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3iyn is a 20 chain structure with sequence from Human adenovirus 5. The December 2010 RCSB PDB Molecule of the Month feature on Adenovirus by David Goodsell is 10.2210/rcsb_pdb/mom_2010_12. Full crystallographic information is available from OCA.