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3hux
From Proteopedia
Contents |
Structure of EF-P bound to the 70S ribosome; THIS FILE CONTAINS THE 50S SUBUNIT FOR MOLECULE I.
Elongation factor P (EF-P) is an essential protein that stimulates the formation of the first peptide bond in protein synthesis. Here we report the crystal structure of EF-P bound to the Thermus thermophilus 70S ribosome along with the initiator transfer RNA N-formyl-methionyl-tRNA(i) (fMet-tRNA(i)(fMet)) and a short piece of messenger RNA (mRNA) at a resolution of 3.5 angstroms. EF-P binds to a site located between the binding site for the peptidyl tRNA (P site) and the exiting tRNA (E site). It spans both ribosomal subunits with its amino-terminal domain positioned adjacent to the aminoacyl acceptor stem and its carboxyl-terminal domain positioned next to the anticodon stem-loop of the P site-bound initiator tRNA. Domain II of EF-P interacts with the ribosomal protein L1, which results in the largest movement of the L1 stalk that has been observed in the absence of ratcheting of the ribosomal subunits. EF-P facilitates the proper positioning of the fMet-tRNA(i)(fMet) for the formation of the first peptide bond during translation initiation.
Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome., Blaha G, Stanley RE, Steitz TA, Science. 2009 Aug 21;325(5943):966-70. PMID:19696344
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3hux is a 31 chain structure with sequence from Thermus thermophilus. The January 2010 RCSB PDB Molecule of the Month feature on 70S Ribosomes by David Goodsell is 10.2210/rcsb_pdb/mom_2010_1. Full crystallographic information is available from OCA.
See Also
- Ribosomal protein L1
- Ribosomal protein L13
- Ribosomal protein L14
- Ribosomal protein L15
- Ribosomal protein L16
- Ribosomal protein L17
- Ribosomal protein L18
- Ribosomal protein L19
- Ribosomal protein L2
- Ribosomal protein L20
- Ribosomal protein L21
- Ribosomal protein L22
- Ribosomal protein L23
- Ribosomal protein L24
- Ribosomal protein L25
- Ribosomal protein L27
- Ribosomal protein L28
- Ribosomal protein L29
- Ribosomal protein L3
- Ribosomal protein L30
- Ribosomal protein L31
- Ribosomal protein L32
- Ribosomal protein L33
- Ribosomal protein L34
- Ribosomal protein L35
- Ribosomal protein L36
- Ribosomal protein L4
- Ribosomal protein L5
- Ribosomal protein L6
- Ribosomal protein L9
- Ribosome
Reference
- Blaha G, Stanley RE, Steitz TA. Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome. Science. 2009 Aug 21;325(5943):966-70. PMID:19696344 doi:325/5943/966
Categories: 70S Ribosomes | RCSB PDB Molecule of the Month | Thermus thermophilus | Blaha, G. | Stanley, R E. | Elongation factor | Initiation | L1-stalk | Metal-binding | Methylation | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Rna-binding | Rrna-binding | Translation | Translation regulation | Trna-binding
